Anti-Human ErbB2 Recombinant Antibody (TAB-005) (CAT#: TAB-005)

• Immunogen
• NIH 3T3/HER2-3400 cells are used to immunize BALB/c mice.

• Host Species
• Mouse

• Derivation
• Humanized (from mouse)

• Type
• IgG1 - kappa

• Specificity
• Tested positive against native human antigen

• Species Reactivity
• Human

• Applications
• FC, IP, ELISA, Neut, FuncS, IF, IHC

• MW
• 145,531.5 g/mol

• Related Disease
• Breast cancers overexpressing ERBB2, metastatic

• Purity
• >95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.

• Storage
• 4°C. For long term storage, aliquot and store at -20°C. Repeated thawing and freezing must be avoided.

• Application Notes
• The ERBB2 antibody has been reported in applications of WB, Inhib, IP, H&E staining, IF, Internalization, ADCC.
  WB: Protein samples lysed in Laemmlli sample buffer and were then boiled for 5 min. Equal quantities of protein lysates were then analyzed on SDS-PAGE and transferred to PVDF membrane. The membranes were blocked with 10% skim milk, incubated with primary antibody in 0.4% skim milk/TBS for 2h, washed with TBST followed by appropriate secondary antibody in 0.4% skim milk/TBS incubation for 1 h. The proteins were visualized using Western lighting plus-ECL reagent in Light-Capture II cooled CCD camera system.
  IF: For confocal microscopic observation, SKBR-3 cells and caveolin-1 transfected SKBR-3 cells were grown on 18 mm cover slips. Cells were then fixed with 4% PFA for 10 min, permeabilized with 0.1% Triton X-100/PBS and blocked with 1% BSA-PBS for 30 min at RT. For caveolin-1 localization, cells were incubated with 1:500 diluted anti-caveolin-1 antibody in 0.25% BSA/PBS for 1 h at RT, followed by staining with Alexa 555 labeled anti-rabbit IgG for 30 min. After wash, the cover slips were mounted with DAPI (Vector Laboratories and observed by confocal microscope IX81 with 60x magnification lens.
  ADCC: Caveolin-1 expressing and wild type SKBR-3 and SKOV-3 cells that had been cultured in 96 well plates were incubated with 1 µM of EC-Fc and Trastuzumab for 4 h at 37˚C.

• Alternative Names
• NEU; NGL; HER2; TKR1; CD340; HER-2; VSCN2; MLN 19; MLN-19; c-ERB2; c-ERB-2; HER-2/neu; p185 (erbB2)

• Gene ID
• 2064

• UniProt ID
• P04626

Select a product category from the dropdown menu below to view related products.