Anti-Human ErbB2 Recombinant Antibody (TAB-005)

Name: Anti-Human ErbB2 Recombinant Antibody (TAB-005)
SKU: TAB-005
Rating: 5 (1 reviews)

CAT#: TAB-005

Recombinant monoclonal antibody to Human ErbB2. The antibody is a monoclonal antibody that interferes with the HER2/neu receptor. Its main use is to treat certain breast cancers.
The HER receptors are proteins that are embedded in the cell membrane and communicate molecular signals from outside the cell (molecules called EGFs) to inside the cell, and turn genes on and off. The HER proteins stimulate cell proliferation. In some cancers, notably certain types of breast cancer, HER2 is over-expressed, and causes cancer cells to reproduce uncontrollably.

Tested Data Gene Expression

Figure 1 Anti-ErbB2 Antibody (TAB-005) in WB

Western blot analysis of TAB-005 was performed with human ErbB2 protein.

ELISA

Figure 2 Anti-ErbB2 Antibody (TAB-005) in ELISA

ELISA analysis of TAB-005 was performed by coating with human ErbB2 protein.

Figure 3 Anti-ErbB2 Antibody (TAB-005) in Dot Blot

Dot Blot analysis of TAB-005 was performed by coating with human ErbB2 protein.

SEC-HPLC

Figure 4 Anti-Human ErbB2 Recombinant Antibody (Trastuzumab) (TAB-005) in HPLC

The purity of TAB-005 was greater than 95% as determined by SEC-HPLC.

SDS-PAGE

Figure 5 Anti-Human ErbB2 Recombinant Antibody (Trastuzumab) (TAB-005) in SDS-PAGE

SDS-PAGE analysis of TAB-005 in reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

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Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg

Normal Tissue Figure 3 High expression in esophagus

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg

Normal Tissue Figure 4 IHC staining of human rectum

Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg

Normal Tissue Figure 5 IHC staining of human urinary bladder

Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg

Normal Tissue Figure 6 Kidney

Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg

Normal Tissue Figure 7 Breast

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2

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Specifications

Immunogen
NIH 3T3/HER2-3400 cells are used to immunize BALB/c mice.

Host Species
Mouse

Derivation
Humanized (from mouse)

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen

Species Reactivity
Human

Applications
FC, IP, ELISA, Neut, FuncS, IF, IHC

MW
145,531.5 g/mol

Related Disease
Breast cancers overexpressing ERBB2, metastatic

Product Property

Purity
>95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.

Storage
4°C. For long term storage, aliquot and store at -20°C. Repeated thawing and freezing must be avoided.

Applications

Application Notes
The ERBB2 antibody has been reported in applications of WB, Inhib, IP, H&E staining, IF, Internalization, ADCC.
WB: Protein samples lysed in Laemmlli sample buffer and were then boiled for 5 min. Equal quantities of protein lysates were then analyzed on SDS-PAGE and transferred to PVDF membrane. The membranes were blocked with 10% skim milk, incubated with primary antibody in 0.4% skim milk/TBS for 2h, washed with TBST followed by appropriate secondary antibody in 0.4% skim milk/TBS incubation for 1 h. The proteins were visualized using Western lighting plus-ECL reagent in Light-Capture II cooled CCD camera system.
IF: For confocal microscopic observation, SKBR-3 cells and caveolin-1 transfected SKBR-3 cells were grown on 18 mm cover slips. Cells were then fixed with 4% PFA for 10 min, permeabilized with 0.1% Triton X-100/PBS and blocked with 1% BSA-PBS for 30 min at RT. For caveolin-1 localization, cells were incubated with 1:500 diluted anti-caveolin-1 antibody in 0.25% BSA/PBS for 1 h at RT, followed by staining with Alexa 555 labeled anti-rabbit IgG for 30 min. After wash, the cover slips were mounted with DAPI (Vector Laboratories and observed by confocal microscope IX81 with 60x magnification lens.
ADCC: Caveolin-1 expressing and wild type SKBR-3 and SKOV-3 cells that had been cultured in 96 well plates were incubated with 1 µM of EC-Fc and Trastuzumab for 4 h at 37˚C.

Target

Alternative Names
NEU; NGL; HER2; TKR1; CD340; HER-2; VSCN2; MLN 19; MLN-19; c-ERB2; c-ERB-2; HER-2/neu; p185 (erbB2)

Gene ID
2064

UniProt ID
P04626

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(Creative Biolabs Cat# TAB-005, RRID: AB_3111733)

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Publications

Peer-reviewed Publications

Our products have been used extensively by scientists all over the world to accelerate their life science research. Here, we present a collection of publications that cite this product.

Nouadje, Georges, and Thierry Ligneel. "Improved immunofixation electrophoresis method with target component on-gel immunodisplacement." U.S. Patent Application No. 16/320,521.

Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-005.pdf

MSDS

MSDS - TAB-005.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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Gly-146LC	Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation)	ELISA	Human antibody
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CAT	Product Name	Application	Type
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